Abstract

The presence of pathogenic microorganisms in cut wounds often leads to wound infection. This investigation is aimed at identifying microorganisms associated with cut wound infection. A total of 40 cut wound specimens were collected from patients within the age interval 11-70 years with cut wound infection in the surgical unit of Federal Medical Centre, Yenagoa, and Federal Medical Centre, Asaba. The specimens were analyzed utilizing culture, Gram staining, biochemical analysis, Kirby Bauer disc diffusion technique, and molecular technique using DNA sequencing for confirmation of the isolates.The bacterial isolates identified were Bacillus sp. 1(6.67%), Escherichia coli 1(6.67%), Klebsiella pneumoniae 2(13.3%), Pseudomonas aeruginosa 3(20%) and Staphylococcus aureus 3(20%), while the fungal isolates were Aspergillus japinocus 1(6.67%), Candida albicans 2(13.3%), Histoplasma capsulatum 1(6.67%), and Madurella sp. 1(6.67%). The predominant organisms associated with the cut wound infection were Pseudomonas aeruginosa (20%) and Staphylococcus aureus (20%). Most isolates showed high resistance to antibiotics used in the susceptibility test. Phylogenetic analysis using 16s rRNA sequences identified bacterial isolates; B. cereus, B. thuringiensis, K. pneumoniae, P. aeruginosa, and fungal isolates; C. albicans and A. japinocus. Regular surveillance and molecular profiling of multidrug-resistant organisms are imperative for controlling the dissemination of these pathogens that do not tolerate antibiotics.